Figure 1.

Reduced Expression of miR-195-5p Is Identified in EC Tissues and Cell Lines

(A) Heatmap of 10 differentially expressed miRNAs in the GEO: GSE43732 expression dataset. The y axis represents the differentially expressed miRNAs. The upper right bar refers to color gradation, and each box indicates the expression level of a gene in a sample. (B) Expression of miR-195-5p in EC and normal samples obtained from the GEO: GSE97049 expression dataset. (C) Relative expression of miR-195-5p in EC tumor tissues and their corresponding adjacent normal tissues (n = 53) determined by qRT-PCR. (D) Expression of miR-195-5p in EC cell lines (KYSE70, KYSE-510, EC9706) and HEECs determined by qRT-PCR. *p < 0.05 compared with normal tissues; ^#p < 0.05 compared with HEECs. Data in (B) and (C) are expressed as mean ± standard deviation and compared by a paired t test. Data in (D) are expressed as mean ± standard deviation, compared by a one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated three times.