Figure 5.

miR-374c Counteracts the Role of mmu_circ_0000790 in Promoting the Viability and Migration of Hypoxic PASMCs

(A) Proliferative ability of PASMCs in each group by CCK-8 assay. (B) Expression of PCNA protein in PASMCs in each group by western blot analysis. (C) Apoptosis of PASMCs in each group by TUNEL assay (original magnification, ×400). (D) Migration ability of PASMCs determined by scratch test. **p < 0.01 versus the normoxia group; ^##p < 0.01 versus the oe-NC and oe-circ_0000790 + miR-374c-mimic groups. The data (mean ± standard deviation) among multiple groups were compared with one-way ANOVA, and those among groups at different time points were compared by ANOVA of repeated measurements. The experiment was run in triplicate independently. miR-374c, microRNA-374c; HPH, hypoxic pulmonary hypertension; PASMC, pulmonary artery smooth muscle cell; CCK-8, Cell Counting Kit-8; PCNA, proliferating cell nuclear antigen; TUNEL, terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling; MC, negative control; ANOVA, analysis of variance.