Figure 5.
The tumor microenvironment in reconstituted animals is comparable. Age matched unirradiated animals (control; open circles), Cesium137 irradiated and reconstituted (Cs 9 Gy; open squares), and X-ray irradiated and reconstituted (X-ray 7 Gy; closed circles) animals were implanted with 4 × 106 B16F10 syngeneic tumor cells subcutaneously. 13 d post implantation, blood (A), spleen (B), and tumors (C) were analyzed for the degree of donor cell reconstitution. Absolute cell number is shown for CD11b+ dendritic cells, B220+ B cells, NKp46+ NK cells, CD4+ T cells, CD8+ T cells, Ly6G+ neutrophils, and CD11b+Ly6C+Ly6G- myeloid cells. Data represents mean +/- standard deviation with 10 mice per group.