Figure 1.

Increased plasma adiponectin in obese hypothalamic Pomc-deficient mice. Comparison of body weights and circulating adiponectin levels in Pomc^+/+ and ArcPomc^−/− female (A) and male (B) mice on a congenic C57BL/6 J genetic background at the indicated ages (n = 6–10). (C) Immunoblots of plasma protein from 8 to 9-week-old female Pomc^+/+ and ArcPomc^−/− mice with a congenic C57BL/6J genetic background. Total, high-molecular-weight (HMW), medium-molecular-weight (MMW), and low-molecular-weight (LMW) adiponectin band intensities were quantified using ImageJ (right) (n = 4). Two-tailed unpaired Student's t-tests were used for comparisons. Data shown are the mean ± s.e.m. of biologically independent samples. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.