Figure 5.

Subcutaneous fat was the primary source of elevated plasma adiponectin in the ArcPomc^−/−mice. (A) Immunoblots of total subcutaneous and gonadal fat lysates from 26-week-old female mice (left). Adiponectin band intensity was normalized to vinculin (right) (n = 4). (B) Quantitative PCR analysis of adiponectin mRNA expression in the subcutaneous, gonadal, and intrascapular brown fat (n = 6–8, 8–9 weeks old, female). Quantitative PCR analysis of Rapgef3 (C) and Adrb3 (D) in the subcutaneous and gonadal fat (n = 6–8, 8–9 weeks old, female). Two-tailed unpaired Student's t-tests were used to compare the genotype effects. Data shown are the mean ± s.e.m of biologically independent samples. ∗P < 0.05, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.