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. 2020 Feb 6;35:100954. doi: 10.1016/j.molmet.2020.01.018

Figure 3.

Figure 3

Alterations in the gut barrier permeability in PTP1B WT and PTP1B KO mice during NASH. A) LPS levels in serum after 4 weeks of feeding mice a chow or MCD diet. n = 6–9 mice per group. B) FITC-dextran assay in PTP1B WT and PTP1B KO mice fed a chow or MCD diet for 4 weeks. Data are expressed as percentage of FITC-dextran levels absorbed in serum samples. n = 9–16 animals per experimental group. ∗∗∗p < 0.001 PTP1B WT chow vs PTP1B WT MCD; ###p < 0.001 PTP1B KO chow vs PTP1B KO MCD; ++p < 0.01 PTP1B WT MCD diet vs PTP1B KO MCD diet. C) Representative images of ZO-1 immunostaining in colon samples of PTP1B WT and PTP KO mice. Scale bar 50 μm. D) Effect of the pro-inflammatory environment on TEER values in Caco-2 shScrb and Caco-2 shPtpn1 monolayers. TEER values from Caco-2 shScrb or shPtpn1 cultured in Transwell plates treated with mCM-C or mCM-LPS are represented as percentage of baseline. Data are expressed as mean ± SEM of three independent experiments performed in triplicate. ∗∗∗p < 0.001 Caco-2 shScb mCM-LPS vs mCM-C; ###p < 0.001 Caco-2 shPtpn1 mCM-LPS vs mCM-C.