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. 2020 Feb 6;35:100954. doi: 10.1016/j.molmet.2020.01.018

Figure 4.

Figure 4

Analysis of anatomical and histological parameters in colon samples from PTP1B WT and PTP1B KO mice fed a chow or MCD diet for 4 weeks. A) Colon moisture content is expressed as percentage of water regarding total colon weight. n = 7–8 mice per experimental group. B) Colonic epithelial granuloma areas expressed as percentage of total epithelium area. n = 4 mice per experimental group. C) Representative images of H&E colonic stained sections from PTP1B WT and PTP1B KO mice. Images were taken from 5 μm colon paraffin-embedded sections stained with H&E with an Axio Scan. Z1 (Zeiss). Granuloma areas are marked with a white symbol. D) Mucin layer analysis in colonic samples from mice under NASH conditions. Images show AB (left) (n = 8–16 crypts per experimental group) and PAS (right) stained representative crypts from colonic sections that stain acidic and neutral mucins, respectively (n = 17–24 crypts per experimental group). E) Muc2 (mucin) mRNA levels are shown as fold change referred to the control PTP1B WT fed a chow diet. Data are expressed as mean ± SEM. n = 5–8 mice per experimental group. ∗p < 0.05; ∗∗p < 0.01 PTP1B WT chow vs PTP1B WT MCD; #p < 0.05; ###p < 0.001 PTP1B KO chow vs PTP1B KO MCD.