TABLE 3.
Plasmids used in this study
| Plasmid | Description | Resistance toa: | Source or reference |
|---|---|---|---|
| pCB578 | E. coli-lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array | Erm | 40 |
| pJV114 | Shuttle vector with pCB578 base containing a spacer targeting mntH1 | Erm | This work |
| pCB591 | E. coli-lactobacilli shuttle vector for homologous recombination template cloning | Amp (E. coli) Cm (Lactobacillus) | 40 |
| pJV88 | Shuttle vector with pCB591 base containing the mntH1 gene and 500-bp homology arms on either side | Amp (E. coli) Cm (Lactobacillus) | This work |
| pJV91 | Shuttle vector with pCB591 base containing 500-bp homology arms on either side of mntH1 that serves as a recombination template to generate a clean deletion | Amp (E. coli) Cm (Lactobacillus) | This work |
| pNZ8148 | Broad-host-range LAB expression vector containing NisA promoter and pepN terminator | Cm | Mobitech |
| pEFB021 | pNZ8148 in which NisA promoter has been replaced by the 207-bp native mntH1 promoter followed by mntH1 | Cm | This work |
a
Erm, erythromycin; Amp, ampicillin; Cm, chloramphenicol.