FIG 2.

Binding of Fe²⁺ ions stimulates the activity of AdhR. (A) ATPase activities of AdhR after reconstitution with increasing amounts of Fe²⁺, Mn²⁺, Zn²⁺, and Fe³⁺. (B) Effect of EDTA treatment on ATPase activities of reconstituted AdhR-Fe(II). AdhR was reconstituted with 60 and 120 μM Fe²⁺, respectively, and then subjected to EDTA treatment. Data shown in all figures are means ± the SD (n = 3 independent experiments). Statistical analysis was performed with the unpaired two-tailed Student t test (***, P < 0.001).