Figure 4.

Zymosan-induced lung inflammation was greater in CGD mice and was associated with increased LTB4. WT and CGD mice were challenged with 20 µg IN zymosan. BAL and lung tissues were collected at different time points. (A) Total leukocyte counts from 3 mL BAL fluid. The percentage of PMNs was determined by Cytospin with Wright-Giemsa stain. BAL PMN counts were calculated by Cytospin results. n ≥ 13 in each group from more than 3 independent experiments. (B) Lung cell counts from the right lung inferior lobe. The percentage of CD45⁺ cells and PMNs (CD45⁺Ly6G⁺CD11b⁺) was determined by flow cytometry. CD45⁺ cell and PMN counts were calculated by flow cytometry. n ≥ 3 in each group from more than 3 separate experiments. (C) Formalin-fixed, paraffin-embedded lung sections were stained with hematoxylin and eosin or processed for immunohistochemical study with MPO. Scale bars: 250 µm (left and middle panels); 50 µm (right panel). (D) Quantification of foci and (E) diameter of foci obtained with immunohistochemical staining with MPO of slides of cells from 5 different mice in each group. LTB4 levels in the first milliliter of BAL fluid supernatant (F) and whole-lung homogenate supernatant (G) were measured by ELISA. (F) n ≥ 7 in each group from more than 3 separate experiments; (G) n ≥ 3 in each group from more than 2 separate experiments. (A-B,E-G) Data are means ± standard error of the mean. *P < .05; **P < .01; ***P < .001; ****P < .0001, by Student t test.