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. 2020 Mar 10;15:1677–1691. doi: 10.2147/IJN.S225807

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© 2020 Hei et al.

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Immunofluorescence of tumor slices and flow cytometry analysis showing CD4⁺ and CD8⁺ T cell infiltration.

Notes: (A) Immunofluorescence of tumor slices.The blue signals represent nuclei stained with DAPI. The green signals represent CD4⁺ T cells stained with AF488-CD4⁺ antibodies and the red signals represent CD8⁺ T cells stained with AF647-CD8⁺ antibodies. (Scale bar: 50 μm). (B, C and D) Flow cytometry analysis and (E and F) their corresponding percentage of CD8⁺ and CD4⁺ tumor infiltrating T cells (n = 3, results are means ± S.D.,*P < 0.05,**P < 0.01).

Abbreviations: aPDL1, programmed death ligand 1 monoclonal antibody; CAT, catalase; SSL, sterically stabilized liposome; aPDL1-SSLs, aPDL1 modified immunoliposomes; CAT@aPDL1-SSLs, CAT-loaded immunoliposomes.