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Synergistic effects of Mohawk and mechanical stretching on tenogenic gene expression in teno-iPSCs. Teno-iPSCs were infected with pHAGE-MKX or control pHAGE-GFP for four days, then split and seeded on vitronectin-coated PCL scaffolds for another two days before being subject to mechanical stretching for 18 h (GFP_Str; MKX_Str). Cells seeded on PCL scaffolds without mechanical loading were served as control (GFP_No; MKX-No). Expression of tenocyte-linked genes was determined by qPCR.
