Table 1.
GlycoDeNovo Analysis of EED Spectra of 18 High-Mannose Isomersa
| glycans | number of isomers | Peak no. | number of supporting fragments | rank by the IonClassifier | reconstructed topology |
|---|---|---|---|---|---|
| Man 4 | 3 | peak 1* | 11 | 1 | Hex Hex Hex Hex HexNAc HexNAc |
| peak 2 | 9 | 1 | [Hex] [Hex] Hex Hex HexNAc HexNAc | ||
| peak 3 | 9 | 1 | [Hex Hex] [Hex] Hex HexNAc HexNAc | ||
| Man 5 | 3 | peak 1 | 9 | 1 | [Hex Hex] [Hex Hex] Hex HexNAc HexNAc |
| peak 2 | 9 | 1 | [Hex Hex] [Hex Hex] Hex HexNAc HexNAc | ||
| peak 3 | 10 | 1 | [[Hex] [Hex] Hex] [Hex] Hex HexNAc HexNAc | ||
| Man 6 | 3 | peak 1 | 12 | 1(2) | [Hex Hex Hex] [Hex Hex] Hex HexNAc HexNAc |
| [[Hex] [Hex] Hex] [Hex Hex] Hex HexNAc HexNAc | |||||
| peak 2* | 9 | 1 | [[Hex Hex] [Hex] Hex] [Hex] Hex HexNAc HexNAc | ||
| peak 3* | 10 | 1 | [[Hex Hex] [Hex] Hex] [Hex] Hex HexNAc HexNAc | ||
| Man 7 | 5 | peak 1 | 11 | 1(2) | [Hex Hex Hex] [Hex Hex Hex] Hex HexNAc HexNAc |
| [[Hex] [Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| peak 2 | 11 | 1 | [[Hex Hex] [Hex] Hex] [Hex Hex] Hex HexNAc HexNAc | ||
| peak 3 | 11 | 1 | [[Hex Hex] [Hex] Hex] [Hex Hex] Hex HexNAc HexNAc | ||
| peak 4 | 13 | 1(2) | [Hex Hex Hex] [Hex Hex Hex] Hex HexNAc HexNAc | ||
| [[Hex] [Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| peak 5 | 12 | 1(2) | [Hex Hex Hex] [Hex Hex Hex] Hex HexNAc HexNAc | ||
| [[Hex] [Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| Man 8 | 3 | peak 1* | 8 | 1 | [[Hex Hex] [Hex Hex] Hex] [Hex Hex] Hex HexNAc HexNAc |
| peak 2* | 12 | 1(5) | [Hex Hex Hex Hex] [Hex Hex Hex] Hex HexNAc HexNAc | ||
| [[Hex Hex] [Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex] [Hex Hex Hex Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex] [[Hex Hex] [Hex] Hex] Hex HexNAc HexNAc | |||||
| peak 3 | 12 | 1(5) | [Hex Hex Hex Hex] [Hex Hex Hex] Hex HexNAc HexNAc | ||
| [[Hex Hex] [Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex] [Hex Hex Hex Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex] [[Hex Hex] [Hex] Hex] Hex HexNAc HexNAc | |||||
| Man 9 | 1 | peak 1 | 12 | 1(5) | [[Hex Hex Hex] [Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc |
| [[Hex Hex] [Hex Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex] [[Hex Hex Hex] [Hex] Hex] Hex HexNAc HexNAc | |||||
| [[Hex] [Hex] Hex] [[Hex Hex] [Hex Hex] Hex] Hex HexNAc HexNAc | |||||
| [[[Hex] [Hex] Hex] [Hex] Hex] [Hex Hex Hex] Hex HexNAc HexNAc |
a
Putative topologies are listed in bold. Asterisks denote peaks where topologies can only be reconstructed by invoking the gap option (see text). Reconstructed topologies are listed using a modified condensed linear IUPAC nomenclature, with branching indicated by square brackets.