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. 2020 Mar 13;11:350. doi: 10.3389/fimmu.2020.00350

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Copyright © 2020 Mandatori, Pacella, Marzolla, Mammi, Starace, Padula, Vitiello, Armani, Savoia, Taurino, De Zio, Giampietri, Piconese, Cecconi, Caprio and Filippini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Three representative carotid endarterectomy samples. The image shows sample division strategy: APR (adjacent atherosclerotic plaque region), AP (atherosclerotic plaque). (B) Frequency of CD45, CD4, CD8, and Tregs (in gated CD4+ T cells) in PBMC, APR, and AP from CEA patients (n = 9). (C) Percentage of OX40 in Tconv and Tregs and gMFI of OX40 gated on Tconv and Tregs. (D) gMFI of BODIPY in CD8, Tconv, and Tregs. In all figures, Tregs have been identified as FOXP3+ CD127^low within the CD14– CD8– viability dye– CD4+ CD25+ gate. *P < 0.05, **P < 0.01, ***P < 0.001, by paired t-test, two-tailed.