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. 2020 Mar 13;11:350. doi: 10.3389/fimmu.2020.00350

Figure 2.

Figure 2

(A) Quantification of atherosclerotic plaque composition in vehicle- or aldosterone-treated ApoE-KO: Representative Red Oil O stained cross sections of aortic root in 8- to 10-week-old ApoE-KO mice treated with vehicle (a) or aldosterone (6 μg/mouse/day) (b) and fed an atherogenic diet for 4 weeks. (B) Percentage of CD45, CD4, CD8 Th1, Th17, and Tregs (in gated CD4+ T cells) in spleens and aortic arches from ApoE-KO mice treated or not with aldosterone (n = 8, four mice each group). (C) Absolute number of Th1, Th17, and Tregs calculated on the percentage in (B). (D) gMFI of LC3 in Th1, Th17, and Tregs. In all figures, Th1, Th17, and Tregs have been identified as CXCR3+CCR6–CD4+, CCR6+CXCR3–CD4+, and FOXP3+CD4+, respectively, within the CD8– CD4+ gate. *P < 0.05, **P < 0.01, ***P < 0.001, by unpaired t-test, two-tailed.