Skip to main content
. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Dev Biol. 2018 Sep 5;451(1):86–95. doi: 10.1016/j.ydbio.2018.08.015

Fig. 1.

Fig. 1.

GFP-VASP localizes to filipodia-like protrusions and cell-cell junctions in endothelial cells undergoing sprouting angiogenesis in zebrafish in vivo. (A) GFP-VASP localizes to puncta at tips of filopodia (arrowheads) of ISV endothelial cells. Bar equals 5 μm. (B) bases of filopodia and/or distal lamella regions at cell tips show stable, elongated accumulations of GFP-VASP (arrowhead). Bar equals 1 μm. (C) Along dorsal axis, some ISVs exhibit transient lamellipodia-like protrusions (arrowhead) with GFP-VASP at the leading edge. Bar equals 3 μm. (D) As ISVs meet at the dorsal side and connect to form the lumen, lamellipodia form nascent cell-cell junctions where VASP is enriched (arrowhead). Bar equals 3 μm. (E) In dorsal aorta and patent ISVs, GFP-VASP localizes to cell-cell junctions. Bar equals 20 μm. (F) ISV cells with high levels of GFP-VASP expression (asterisk) show numerous small filopodia and fail to properly migrate to the dorsal junction point. Elapsed time shown in minutes, bar equals 100 μm.