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Alkaline phosphatase (ALP) staining for early mineralization activity induced by 14 days of IGF‐1 treatment in MC3T3‐E1 cells. The cells were cultured in six‐well plates for 14 days with 0, 10, 50, 100, and 200 μg/L. ALP staining showed the expression and enzymatic activity of the phosphatase in MC3T3‐E1 cells. Early phase mineralization was measured using ALP staining
