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The effect of the major compositions of FMC medium on PlrgA activation. The PlrgA-gfp reporter strain was grown in a complete FMC11 (A) and FMC11, lacking carbonate (-carb, B), acetate (-ace, C), or citrate (-cit, D). Relative gfp expression (gray circle) and cell growth (OD600; black diamond) were monitored during growth on a plate reader (see section “Materials and Methods” for details). The results are representative of three independent experiments.
