FIGURE 6.

The effect of different concentrations of potassium on eliciting the PlrgA activation in TV11. The PlrgA-gfp reporter strain was grown in TV11, supplemented by different concentrations of potassium (A, 0 mM; B, 1 mM; C, 5 mM; D, 10 mM; E, 20 mM; and F, 40 mM) as K-phosphate (kTV11). Relative gfp expression (gray circle) and cell growth (OD₆₀₀; black diamond) were monitored during growth on a plate reader (see section “Materials and Methods” for details). The results are representative of three independent experiments.