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. 2020 Mar 13;11:412. doi: 10.3389/fmicb.2020.00412

FIGURE 3.

FIGURE 3

HO-1 specific inhibitor ZnPP partially reverses the inhibitory effect of CoPP on PRV. (A) PK-15 or (B) ST cells were treated with 0, 20, 40, 60 μM of ZnPP for 24 h. Cells were harvested to detect HO-1 protein expression via western blotting. PK-15 or ST cells were treated with 0, 20, 40, and 60 μM of ZnPP for 12 h at 37°C. Then cells were infected with 0.01 MOI of PRV and followed by incubating with 50 μM of CoPP from 1 hpi onwards. (C,D) Cells were collected to detect PRV gB protein expression via western blotting, while (E,F) supernatant progeny copies were assessed via RT-qPCR, at 36 hpi. Data are presented as the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. HO-1, heme oxygenase-1; ZnPP, zinc-protoporphyrin; CoPP, cobalt-protoporphyrin; PRV, pseudorabies virus; RT-qPCR, reverse transcription-quantitative PCR.