FIGURE 4.
Overexpression of HO-1 suppresses PRV replication. PK-15 or ST cells stably expressing HO-1 were verified via (A) RT-qPCR and (B) western blotting. PK-15HO–1 or STHO–1 cells were infected with PRV at a MOI of 0.01. Samples were harvested at 12, 24, 36, 48 hpi. (C,D) Western blotting was performed to assess PRV gB and HO-1 protein expression levels, while (E,F) RT-qPCR was performed to detect supernatant progeny virus copies. Data are presented as the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. HO-1, heme oxygenase-1; PRV, pseudorabies virus; RT-qPCR, reverse transcription-quantitative PCR; MOI, multiplicity of infection.