FIGURE 5.

Knockdown of endogenous HO-1 promotes PRV replication. PK-15^shHO–1 or ST^shHO–1 cells were identified via (A) RT-qPCR and (B) western blotting. PK-15^shNC, PK-15^shHO–1, ST^shNC, and ST^shHO–1 cells were incubated with PRV at a MOI of 0.01. Cells and supernatants were collected at 12, 24, 36, 48 hpi to assess (C,D) PRV gB and HO-1 protein expression levels via western blotting, while (E,F) supernatant virus copies were detected via RT-qPCR (E,F). Data are presented as the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. HO-1, heme oxygenase-1; PRV, pseudorabies virus; sh, short hairpin; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR; MOI, multiplicity of infection.