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. 2020 Mar 20;21:14. doi: 10.1186/s12865-020-00343-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Gating strategy for the different B lymphocyte subtypes from whole blood. The lymphocyte gate was based on FSC and SSC characteristics, followed by a gating of lymphocyte singlets by FSC-H and FSC-A. Plasmablasts (PB) were defined as being CD19 + CD24-CD38++CD27 + CD20-, pre-plasmablasts (pre- PB) as CD19 + CD24-CD38++CD20+ and transitional B cells (TB) as CD19 + CD24++CD38++. The naïve B cells were defined as CD19 + CD27-IgD+ excluding the TB. The switched memory B cells (SM) were defined as CD19 + CD27 + IgD-, the non-switched memory B cells (NSM) were defined as CD19 + CD27 + IgD+, and the double negative memory B cells (DNM) were defined as CD19 + CD27-IgD-. All B cell subtypes were analyzed for CD86 and BTLA expressions, expressed as mean fluorescence intensity (MFI)