Table 1.
Cell line specifications
| Cell line | Seeding concentration | Rituximab | Cyclophosphamide | Doxorubicin | Vincristine | ABC/GCB | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| cells/mL | AUC | AUC | AUC | AUC | ||||||
| DB | 0.125 × 106 | 318.1 | Int | 345.6 | Res | 275.5 | Res | 130.7 | Res | GCB |
| FARAGE | 0.25 × 106 | 344.1 | Int | 311.0 | Res | 179.6 | Sen | 56.1 | Sen | GCB |
| HBL-1 | 0.25 × 106 | 421.9 | Res | 226.0 | Int | 272.2 | Int | 84.5 | Int | ABC |
| MC-116 | 0.25 × 106 | NA | NA | NA | NA | 271.8 | Int | 62.0 | NA* | GCB |
| NU-DHL-1 | 0.125 × 106 | 316.6 | Int | 171.7 | Sen | 200.6 | Sen | NA | NA | ABC |
| NU-DUL-1 | 0.25 × 106 | 236.4 | Sen | 214.1 | Sen | 223.9 | Int | 90.3 | Int | UC |
| OCI-Ly3 | 0.125 × 106 | 407.6 | Res | 261.7 | Int | 253.4 | Int | 74.8 | Int | ABC |
| OCI-Ly7 | 0.125 × 106 | 218.3 | Res | 257.9 | Sen | 324.9 | Res | 114.5 | Int | GCB |
| OCI-Ly8 | 0.25 × 106 | 406.6 | Res | NA | NA | NA | NA | NA | NA | UC |
| OCI-Ly19 | 0.25 × 106 | 400.4 | Res | 202.1 | Sen | 166.9 | Sen | 54.0 | Sen | UC |
| RIVA | 0.25 × 106 | 275.8 | Res | 296.0 | Int | 326.7 | Res | 109.0 | Res | ABC |
| SU-DHL-4 | 0.25 × 106 | 150.4 | Sen | NA | NA | 288.8 | Res | NA | NA | GCB |
| SU-DHL-5 | 0.25 × 106 | 251.2 | Sen | 164.5 | Sen | 201.5 | Sen | 57.9 | Sen | GCB |
| SU-DHL-8 | 0.5 × 106 | 425.0 | Res | 270.6 | Int | 222.0 | Sen | 126.1 | Res | GCB |
| U2932 | 0.4 × 106 | 354.1 | Int | 329.8 | Res | 294.8 | Res | 85.3 | Int | GCB |
For each drug, DLBCL cell lines were ranked according to sensitivity based on area under the dose-response curve (AUC) for rituximab, cyclophosphamide, doxorubicin, and vincristine. Division into tertiles defines: Rituximab, 4 sensitive, 5 intermediate, 5 resistant; Cyclophosphamide, 4 sensitive, 4 intermediate, 4 resistant; Doxorubicin, 5 sensitive, 4 intermediate, 5 resistant; Vincristine, 4 sensitive, 4 intermediate, 4 resistant. Based on GEP, DLBCL cell lines were classified into ABC/GCB subclasses by Wright classification using published algorithms at hemaClass.org [17]. ABC, activated B-cell like; GCB, germinal center B-cell like; Int, intermediate; NA, not available; Res, resistant; Sen, sensitive; * excluded due to large variation between replicates