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. 2020 Mar 20;21:71. doi: 10.1186/s12931-020-1331-4

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Wnt5a levels detecting in hypoxia induced PAECs and PAMSCs. a Immunofluorescence staining with CD31 (red), Wnt5a (green) and the cell nuclei were labelled with DAPI (blue) in the PAECs at a × 400 magnification. b Immunofluorescence staining with a-SMA (green), Wnt5a (red) and DAPI (blue) in PAMSCs at a × 400 magnification. c Protein expression and comparative analysis of of CD31 and a-SMA by Western blot. n = 3 times repeated; P < 0.05, one-way ANOVA followed by post hoc test; the data are present as mean ± SD; ^*hypoxia or ^*MSC-CM vs. control; ^#MSC-exo vs. hypoxia group. Scale bar = 100 μm