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. 2020 Mar 19;8:35. doi: 10.1186/s40478-020-00903-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Hypoxia responses in human CP explants. a Schematic representation of the experimental setup of CP explants cultured in hypoxia and normoxia. b Difference in relative gene expression between human postmortem CP paired samples from each donor (n = 6) incubated 24 h in hypoxia (1% O₂) or normoxia (20% O₂), analyzed using RT-qPCR. Data are normalized to 18 s rRNA. Results are displayed as the negative difference in Ct values between hypoxia and normoxia (represented as -ΔΔCt) and presented as median with confidence interval. Differences were tested by paired two-tailed Welch t-test