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. 2020 Mar 13;8:180. doi: 10.3389/fbioe.2020.00180

Table 1.

Characterization of human amniotic membrane-derived cells.

	Human amniotic epithelial cells (hAECs)	Human amniotic mesenchymal cells (hAMCs)	References
Phenotype of cells	Flat, cuboidal, and columnar epithelial cells	Plastic-adherent, spindle-shaped cells
Positive markers during passages
Embryonic stem cell, self-renewal, and pluripotency markers	TRA1-60^†^×, TRA1-81^†^×, SSEA-3^, SSEA-4^ ^×, OCT4^* ^×, Nanog^* ^×, SOX-2^* ^×, SOX17^* ^×, KLF-4^* ^×, c-MYC^* ^×, REX-1, CFC-1, DPPA-3, PROM-1, PAX-6, FOXD3, GDF3, TfE3	TRA1-60^†^×, TRA1-81^†^×, OCT3, OCT4^* ^×, Nanog^* ^×, SOX-2^* ^×, SOX17^* ^×, KLF-4^* ^×, c-MYC^* ^×	Miki et al., 2005; Ilancheran et al., 2007; Bilic et al., 2008; Ge et al., 2012; Roubelakis et al., 2012; Zhou et al., 2013; Koike et al., 2014; Chen et al., 2015; Garcia-Castro et al., 2015; Samsonraj et al., 2017
Epithelial cell markers	CK-1, CK-2, CK-3, CK-4, CK-5, CK-6, CK-7, CK-8, CK-10, CK-13, CK-14, CK-15, CK-16, CK-19, CA-125, MUC-16, CD326^×^(EpCAM), CD324^ (E-Cadherin), CD73	CD324^, CD326^×^(EpCAM)	Nanbu et al., 1989; Diaz-Prado et al., 2011; Pratama et al., 2011; Caruso et al., 2012; Paracchini et al., 2012; Centurione et al., 2018
Mesenchymal cell markers	CD73^, CD271^, CD24^†^, CD90^†^, CD133^*^†, CD44^†^×	CD73^, CD271^, CD24^, CD90^, CD133^*, CD105^×	Soncini et al., 2007; Murphy et al., 2010; Roubelakis et al., 2012; Iaffaldano et al., 2013; Sivasubramaniyan et al., 2013; Koike et al., 2014; Spitzhorn et al., 2017; Schmelzer et al., 2019
Human leukocyte antigens	HLA-A^, HLA-B^ HLA-C^, HLA-DR^	HLA-A^, HLA-B^, HLA-C^*	Bilic et al., 2008; Fatimah et al., 2010; Koike et al., 2014; Magatti et al., 2015, 2016; Pogozhykh et al., 2015
Specific cell lineage and functional markers	Apo-D, A2B5^, MMP-1, PDGF Receptor ß (CD140b), Musashi-1, Nestin^, Vimentin^* ^×, PSA-NCAM, β-tubulin-III, Catecholamine, Norepinephrine, Dopamine, DOPAC, Choline acetyltransferase (ChAT), Acetylcholine, GATA-4^, Hepatocyte nuclear factor-3ß, AFP^×, Albumin^, Glucose-sensing molecule (GLUT-2), Insulin, RCI^, Neurofilament proteins^, MAP2 kinase^, Microtubule-associated protein 2^ (MAP2), Glial fibrillary acidic protein^, CNPase^, Myelin basic protein^, Galactocerebroside^, Atrial myosin light chain- 2^* (MLC-2A), Ventricular myosin light chain- 2^* (MLC-2V), Nkx 2.5^, α-actinin, collagen type II^, Osteocalcin^, Osteopontin^, ALP^*, Type I collagen	CD133, Nestin^, Albumin^, α-fetoprotein^× (α-FP), Cytokeratin 18 (CK18), α1-Antitrypsin (α1-AT), Hepatocyte nuclear factor 4α (HNF4α), PDX-1, RCI^, A2B5^, Neurofilament proteins^, MAP2 kinase^, Microtubule-associated protein 2^* (MAP2), Glial fibrillary acidic protein^, CNPase^, Myelin basic protein^, Galactocerebroside^, Atrial myosin light chain- 2^* (MLC-2A), Ventricular myosin light chain- 2^* (MLC-2V), GATA-4^, Nkx 2.5^, α-actinin, collagen type II^, Osteocalcin^, Osteopontin^, ALP^, type I collagen^*	Sakuragawa et al., 1996, 2000, 2004; Elwan and Sakuragawa, 1997; Kakishita et al., 2000; Takahashi et al., 2001, 2002; Wei et al., 2003; Portmann-Lanz et al., 2006; Kim et al., 2007; Gomez Dominguez, 2008; Kong et al., 2008; Parolini et al., 2008; Tamagawa et al., 2008; Manuelpillai et al., 2011; Miki, 2011; Niknejad et al., 2012; Alcaraz et al., 2013; Fatimah et al., 2013; Koike et al., 2014; Garcia-Lopez et al., 2015; Sarvandi et al., 2015; Bollini et al., 2018; Centurione et al., 2018; Liu et al., 2018; Maymo et al., 2018
Other markers	CD1b,CD9^, CD10, CD13^†, CD24, CD26, CD29^,CD31, CD34, CD46, CD49a, CD49b, CD49c, CD49d, CD49e^, CD49f, CD55, CD58, CD59, CD63, CD77, CD81,CD83, CD91, CD95, CD98, CD104, CD109, CD117^×^, CD 133, CD142, CD144,CD146, CD147, CD151, CD164,CD166^*, CD227, ABCG2/BCRP	CD9^, CD13^, CD27^†, CD29^, CD31, CD49e^, CD54, CD166^, CD117^ ^×, CD349, Vimentin^* ^×, STRO-1, BMP-4	Gomez Dominguez, 2008; Fatimah et al., 2010; Murphy et al., 2010; Pratama et al., 2011; Zhou et al., 2013; Koike et al., 2014; Pozzobon et al., 2014; Magatti et al., 2015
Negative markers during passages	CD11,CD14^, CD31^, CD62, CD349, HLA-A2, VWF	CD3, CD14^*, CD34, CD45, CD324 (E-cadherin), HLA-DR^×, HLA-DP, HLA-DQ	Miki et al., 2005; Portmann-Lanz et al., 2006; Ilancheran et al., 2007; Wolbank et al., 2007; Bilic et al., 2008; Murphy et al., 2010; Pratama et al., 2011; Magatti et al., 2012, 2016; Koike et al., 2014; Alikarami et al., 2015; Si et al., 2015; Phermthai et al., 2017; Samsonraj et al., 2017

^*

Expressed on both hAECs and hAMSCs.

^†

Low expression.

^×

Discrepancy witnessed.