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. 2020 Mar 20;11:127. doi: 10.1186/s13287-020-01628-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Isolation, identification, and characterization of hSMGepiS/PCs. a Images of primary cells isolated and passaged in 2D culture. Scale bar = 200 μm. b Representative images of single cells 3D-cultured and spheres formed. Scale bar = 1 mm (above) and 200 μm (below). c Quantification of sphere formation of hSMGepiS/PCs from different donors at day 7 after 3D culture. Error bars, SD, n = 6. d Immunofluorescence of K19 in cells and hSMGs to locate the isolated cells. Scale bar = 100 μm. e Positive expression of an epithelial marker, E-cadherin, in isolated cells. Scale bar = 100 μm. f Immunofluorescence showing that isolated cells express the proliferation marker, Ki67, and the epithelial stem/progenitor cell markers, K5 and CD49f. Scale bar = 100 μm. g Protein levels of the indicated markers in hSMGepiS/PCs and hSMGMCs by western blotting