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. 2020 Mar 20;11:127. doi: 10.1186/s13287-020-01628-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Long-term culture of hSMGepiS/PCs. a Images of long passaged hSMGepiS/PCs in 2D culture. Scale bar = 200 μm. b Growth curve and c doubling time of passaged hSMGepiS/PCs in 2D culture. Error bars, SD. Statistical analysis was performed using ANOVA, F = 1.266, P = 0.357, n = 3. d qRT-PCR showing that molecular markers of stem/progenitor cells were intact after long-term culture. Error bars, SD. ANOVA, F = 1.820 and P = 0.222 (K5), F = 1.183 and P = 0.383 (CD49f), F = 1.467 and P = 0.295 (Ascl3), n = 3. e Bulk DNA-seq results of hSMGepiS/PCs at passages 1, 8, and 16. Chromosomes are indicated by interlaced colors. Points are bins of 200 kb length