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. 2020 Mar 13;11:317. doi: 10.3389/fphar.2020.00317

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Copyright © 2020 Guo, Li, Qiu, Liu, Qin, Hou and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell uptake and intracellular distribution of NPs/10-Hydroxycamptothecin (HCPT). (A) Confocal microscopy images of B16F10 cells after incubation with free HCPT or NPs/HCPT for 2 h (scale bar, 20 μm). (B) Confocal microscopy images of B16F10 cells after incubation with free HCPT or NPs/HCPT for 6 h (scale bar, 20 μm). (C) Quantitative analysis of optical density of HCPT fluorescence intensity after 2 h or 6 h of treatment with free HCPT or NPs/HCPT. (D) Cell uptake of B16F10 cells treated with free HCPT or NPs/HCPT at a HCPT concentration of 1.5 μg ml⁻¹. Data are presented as mean ± SD (n = 3; *P < 0.05).