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. Author manuscript; available in PMC: 2021 Jan 23.
Published in final edited form as: Cell. 2019 Dec 26;180(2):340–347.e9. doi: 10.1016/j.cell.2019.12.003

Figure 3. Structure of hKCNQ1EM-KCNE3-CaM complex in the presence of PIP2.

Figure 3.

(A) Liposome based flux assay. Recordings (n=3) from PE:PG:PIP2 alone, PE:PG with hKCNQ1EM-KCNE3-CaM and PE:PG:PIP2 with hKCNQ1EM-KCNE3-CaM vesicles are plotted in black, blue and green traces.

(B) SDS-PAGE showing the reconstitution of hKCNQ1EM-KCNE3-CaM complex in nanodiscs containing PIP2 using MSP2N2 as the scaffold protein.

(C) Cryo-EM map and structure model of hKCNQ1EM-KCNE3-CaM in the presence of PIP2. KCNQ1, KCNE3 and CaM are colored in blue, red and orange, respectively.