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. 2020 Mar 13;10:97. doi: 10.3389/fcimb.2020.00097

Figure 4.

Figure 4

Identification of dysregulated miRNAs. (A) Heatmap and clustering analysis of DE miRNAs. Each row represents one miRNA and each column represents one sample; −2, −1, 0, 1, and 2 represent fold change. Red indicates high expression and blue represents low expression. CON-1, CON-2, and CON-3 represent three mock-infected samples; HTNV-1, HTNV-2, and HTNV-3 represent three HTNV-infected samples. (B) DE miRNAs count. (C) Verification of DE miRNAs upon HTNV infection. HUVECs were infected with HTNV 76-118 (MOI = 1) for 3 days. Then the total RNA was extracted and the expression levels of miRNAs were measured by RT-qPCR. Student's t-test, mean ± SD, ***P < 0.001. The experiment was performed at least three times independently. (D) GO functional enrichment analysis of target genes of DE miRNAs. The x-axis shows the P-value and gene numbers, and the y-axis shows the GO term. (E) KEGG pathway analysis revealed the top 10 enriched pathways of target genes of DE miRNAs.