Table 5.

Concordance of mutation results between fresh frozen and FFPE tissues, at the gene level.

Mutation Genes	a*	b*	c*	d*	Concordance#	Kappa	P
AKT1	1	0	1	116	99.2%	0.663	<0.001
BRAF	1	0	2	116	99.2%	0.796	<0.001
CTNNB1	0	0	1	118	100.0%	1.000	<0.001
DDR2	1	0	1	116	99.2%	0.663	<0.001
EGFR	1	1	0	114	98.3%	−0.009	0.925
ERBB2	0	0	1	105	100.0%	1.000	<0.001
ERBB4	2	1	2	113	97.5%	0.559	<0.001
FBXW7	5	3	13	64	90.6%	0.706	<0.001
FGFR2	1	0	2	113	99.1%	0.796	<0.001
KRAS	16	8	46	92	85.2%	0.679	<0.001
MAP2K1	1	0	4	99	99.0%	0.884	<0.001
NRAS	3	3	2	106	94.7%	0.372	<0.001
PIK3CA	10	12	18	90	83.1%	0.512	<0.001
PTEN	5	2	6	57	90.0%	0.575	<0.001
SMAD4	5	2	6	111	94.4%	0.602	<0.001
TP53	10	35	54	73	73.8%	0.481	<0.001

^*a, Mutation identified in fresh frozen tissue, but not in FFPE tissue; b, Mutation identified in FFPE tissue, but not in fresh frozen tissue; c, Mutation identified in FFPE and fresh frozen tissue; d, Mutation was not identified in FFPE or fresh frozen tissue;

^#Concordance = (c+d)/(a+b+c+d).