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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Allergy. 2019 Jul 25;74(12):2449–2460. doi: 10.1111/all.13968

Figure 1: Esophageal epithelial cells express high levels of TLR2 and 3.

Figure 1:

TLR expression was determined by qRT-PCR. A) Primary esophageal epithelial cells from EoE patients and controls express TLR1-6, CD14, CLEC7A (Dectin-1); mean ±SD, n=3 biologic replicates. TLR7-10 not shown, resulted as not amplified. B) TLR1-6, TLR9, CD14, and CLEC7A (Dectin1) are expressed on immortalized epithelial cell line EPC2-hTERT; mean ±SD, n=3. TLR7, 8 and 10 not amplified. C) TLR2 surface expression on EPC2-hTERT cells was assessed by flow cytometry, and D) the median fluorescence intensity is shown (**p<0.001 by t-test, n=3). E) EPC2-hTERT cells evaluated for expression of Dectin-1 by flow cytometry.