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. 2020 Mar 13;10:294. doi: 10.3389/fonc.2020.00294

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Copyright © 2020 Li, Peng, Yang, Chen, Gu, Qian, Ji, Wang, Zhang, Tang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-1224-5p mediates the promoting effects of hypoxia on metastasis and EMT of CRC cells. (A) The expressions of HIF-1α in different time points in normoxia and hypoxia condition. (B) The levels of miR-1224-5p in SW480 cells cultured in normoxia and hypoxia. (C,D) Transwell assays and Wound healing assay revealed that hypoxia promoted migration and invasion of SW480 cells, while miR-1224-5p overexpression abolished the effects of hypoxia, scale bars: 100 μm (C) and 300 μm (D,E). Hypoxia facilitated the EMT process of SW480 cells and miR-1224-5p restoration showed an opposite effect. (F) IκBα, phosphorylated IκBα, p65, and phosphorylated p65 protein levels in whole cell lysate, cytosol and nucleus were analyzed by Western blot. Data represent the mean ± SD from three independent experiments. Student's t-test was used to determine statistical significance: *p < 0.05, and **p < 0.01.