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. 2020 Mar 13;10:294. doi: 10.3389/fonc.2020.00294

Figure 3.

Figure 3

miR-1224-5p inhibits the EMT process of CRC cells. (A) Western blot analysis of tight junction protein ZO-1, epithelial marker E-cadherin, and mesenchymal markers N-cadherin and Vimentin after miR-1224-5p knockdown in SW480 cells. (B) IF staining of E-cadherin and Vimentin after miR-1224-5p knockdown in SW480 cells, scale bars: 10 μm. (C) miR-1224-5p overexpression increased ZO-1 and E-cadherin expression, and decreased the levels of N-cadherin and Vimentin in HCT116 cells. (D) IF staining of E-cadherin and Vimentin after miR-1224-5p overexpression in HCT116 cells, scale bars: 10 μm. (E) Immunohistochemistry of E-cadherin and Vimentin were detected and compared between miR-1224-5p high expressing CRC tissues and miR-1224-5p low expressing cases. Data represent the mean ± SD from three independent experiments. Student's t-test was used to determine statistical significance: *p < 0.05.