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. 2020 Mar 13;10:294. doi: 10.3389/fonc.2020.00294

Figure 4.

Figure 4

SP1 was proved to be a potential target of miR-1224-5p. (A) Luciferase reporter assay was conducted to verify that miR-1224-5p bound to the 3′-UTR region of SP1 directly. miR-1224-5p overexpression significantly suppressed, while miR-1224-5p loss increased the luciferase activity that carried wild-type (wt) but not mutant (mut) 3′-UTR of SP1. (B) miR-1224-5p knockdown increased the expression of SP1 mRNA and protein in SW480 cells and miR-1224-5p overexpression decreased the level of SP1 mRNA and protein in HCT116 cells, respectively. Data represent the mean ± SD from three independent experiments. Student's t-test was used to determine statistical significance: *p < 0.05 and **p < 0.01.