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. 2020 Mar 13;10:294. doi: 10.3389/fonc.2020.00294

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Copyright © 2020 Li, Peng, Yang, Chen, Gu, Qian, Ji, Wang, Zhang, Tang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-1224-5p inhibits migration and invasion by targeting SP1. (A) Expression of SP1 mRNA and protein was verified in transfected CRC cell lines by qRT-PCR and western blot. (B) Expression of SP1 was confirmed by qRT-PCR and western blot in co-transfected CRC cell lines. (C,E) Wound healing assay and Transwell assays confirmed that SP1 knockdown abrogated the effects of miR-1224-5p loss on migration and invasion of SW480 cells, scale bars: 300 μm (C) and 100 μm (E). (D,F) SP1 restoration promoted migration and invasion of miR-1224-5p-overexpressing of HCT116 cells, scale bars: 300 μm (D) and 50 μm (F). Data represent the mean ± SD from three independent experiments. Student's t-test was used to determine statistical significance: *p < 0.05, **p < 0.01, and ***p < 0.001.