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. 2020 Mar 13;10:294. doi: 10.3389/fonc.2020.00294

Figure 8.

Figure 8

NF-κB signaling is essential for the biological function of miR-1224-5p in CRC. (A) SW480 and HCT116 cells that were transfected with corresponding miRNA lentivirus were subjected to immunoblotting for SP1, IκBα, phosphorylated IκBα, p65, phosphorylated p65, and EMT. (B,C) NF-κB inhibitor HY-10257 treatment abrogated the effect of miR-1224-5p loss on mobility of HCT116 and SW480 cells, scale bars: 50 μm (B) 100 μm (C,D) Western blot analysis indicated that modulating p65 phosphorylation reversed the effects of miR-1224-5p alteration on EMT process of CRC cells. (E) Expression of protein in co-transfected and transfected CRC cells was detected by western blot. (F) IκBα, phosphorylated IκBα, p65 and phosphorylated p65 protein levels in whole cell lysate, cytosol and nucleus were analyzed by Western blot. Data represent the mean ± SD from three independent experiments. Student's t-test was used to determine statistical significance: *p < 0.05, ***p < 0.001.