Table 4. List of selected characteristics of the data distributions from each of the peptide array formats.
The table below lists seven different numerical descriptors of the full dataset from each peptide microarray library (330K short peptide, 330K long peptide, 125K short peptide, 124K short peptide), and for each antibody. In order, the descriptors are: Shannon’s Entropy [17], 95th percentile coefficient of variance (calculated from the standard deviation / mean of the 95th upper percentile of all observed fluorescence intensities for each antibody per peptide library), the mean of all intensities, the stdev of all intensities, the measured kurtosis of all raw intensities, the skewness of the raw intensity data distribution, and the dynamic range of the raw intensities.
| Antibody | CIM330K short | CIM330K short | CIM330K short | CIM330K short | CIM330K short | CIM330K short | CIM330K short |
| Entropy | 95th%ile var | mean | stdev | kurtosis | skewness | DR | |
| AKT1(B-1) | 6.59699095 | 0.94827464 | 997.931681 | 663.127174 | 2854.00735 | 34.8141055 | 4.97326203 |
| AKT1(7) | 6.40821724 | 0.43249303 | 742.91363 | 603.430384 | 5633.04555 | 55.6273088 | 6.21982759 |
| Akt2(D-17) | 6.34880064 | 0.16847798 | 872.804152 | 612.759423 | 3395.00949 | 38.431947 | 5.66894198 |
| p53 Ab1 | 6.43404063 | 0.89970233 | 839.422526 | 749.430333 | 3689.28369 | 47.0647225 | 6.72727273 |
| p53 Ab8 | 6.67474666 | 0.58985062 | 743.369711 | 720.764303 | 3992.68735 | 50.5967494 | 5.98739496 |
| DM1A | 6.58937783 | 0.35993306 | 1000.65025 | 955.97848 | 2427.36127 | 40.1117727 | 4.96216216 |
| JNK2 | 6.57656755 | 0.16700709 | 960.27086 | 700.313264 | 2740.54469 | 35.8329888 | 5.55246914 |
| hnRNP-A1 | 7.00998041 | 0.77525392 | 847.922562 | 548.851338 | 2770.05958 | 27.4521631 | 7.05416667 |
| FDFT1 | 6.5936509 | 0.42609586 | 938.274437 | 1196.20813 | 1629.28596 | 34.6819536 | 6.56183746 |
| p21 | 6.45104349 | 0.46370076 | 1569.97092 | 1591.95415 | 630.053602 | 18.1557846 | 9.49470899 |
| p-p21 | 6.48341516 | 0.5038796 | 1857.37495 | 4592.18497 | 107.793395 | 9.53785444 | 10.1113924 |
| p27 | 6.37471234 | 0.9331044 | 718.065902 | 866.060381 | 2911.37616 | 44.2815305 | 10.1081081 |
| p-p27 | 7.31495785 | 0.68156289 | 746.468132 | 551.025209 | 2665.77271 | 30.1878272 | 7.95336788 |
| p38 | 6.46027583 | 0.31336035 | 727.846199 | 588.212965 | 5505.69919 | 54.531433 | 6.19736842 |
| p-p38 | 6.5446979 | 0.09138093 | 983.501078 | 2071.57046 | 700.793202 | 24.828498 | 5.57377049 |
| p-ERK | 6.44468899 | 0.70707617 | 913.976836 | 890.949155 | 2365.7115 | 37.6924918 | 6.92015209 |
| Cyclin B1 | 6.64415963 | 0.50225685 | 912.835678 | 826.054839 | 3050.90059 | 43.6578988 | 5.60128617 |
| IFN-y | 6.45587552 | 0.34102307 | 826.167115 | 544.492649 | 4009.04013 | 36.653937 | 6.69262295 |
| biotin IFN-y | 6.42640065 | 0.13482229 | 1244.56755 | 1613.80366 | 563.224489 | 18.281687 | 8.80906149 |
| 6A7 Bax | 6.69588997 | 0.75509245 | 912.086591 | 1216.86118 | 2346.99294 | 45.0626643 | 5.07854985 |
| Anti-BrdU | 7.87462317 | 0.20935228 | 2624.61493 | 6649.98001 | 51.3294253 | 6.52958368 | 31.1350575 |
| SR 1H4 | 6.43766881 | 0.66607249 | 763.409327 | 685.3969 | 4534.99939 | 52.4777171 | 6.48695652 |
| Clk1 | 6.45874743 | 0.8520575 | 759.433941 | 618.395681 | 5340.39725 | 54.3463068 | 6 |
| LnkB B2 | 6.76805579 | 0.14024813 | 759.700558 | 496.349923 | 578.518773 | 9.01597547 | 10.6933333 |
| Antibody | CIM330K long | CIM330K long | CIM330K long | CIM330K long | CIM330K long | CIM330K long | CIM330K long |
| Entropy | 95th%ile var | mean | stdev | kurtosis | skewness | DR | |
| AKT1(B-1) | 6.59699095 | 0.28557628 | 4554.77322 | 1300.73519 | 622.307065 | 13.5654982 | 2.14203317 |
| AKT1(7) | 6.40821724 | 0.21273616 | 4104.46845 | 873.168854 | 838.54933 | 14.029646 | 1.84681881 |
| Akt2(D17) | 6.34880064 | 0.1995204 | 4411.52709 | 880.18965 | 1240.02572 | 19.8666298 | 1.69823386 |
| p53 Ab1 | 6.43404063 | 0.24515575 | 4647.1139 | 1139.2667 | 1048.33146 | 21.9729809 | 1.73474044 |
| p53 Ab8 | 6.67474666 | 0.3634331 | 4424.55606 | 1608.03013 | 286.62671 | 11.3306855 | 2.10854583 |
| DM1A | 6.58937783 | 0.36094758 | 4415.52644 | 1593.77357 | 563.688905 | 15.318917 | 2.61970256 |
| JNK2 | 6.57656755 | 0.32165721 | 4352.02409 | 1399.85992 | 506.28591 | 12.0138395 | 2.74750357 |
| hnRNP-A1 | 7.00998041 | 0.38475586 | 5347.05528 | 2057.31086 | 93.9357374 | 5.06685171 | 2.65914319 |
| FDFT1 | 6.5936509 | 0.29834674 | 4274.13257 | 1275.17352 | 361.444301 | 7.32255294 | 2.7878202 |
| p21 | 6.45104349 | 0.24655935 | 4927.17232 | 1214.84041 | 893.585872 | 21.018091 | 1.69355698 |
| p-p21 | 6.48341516 | 0.44809185 | 4725.45688 | 2117.43872 | 648.486626 | 23.0610095 | 1.78139269 |
| p27 | 6.37471234 | 0.211287 | 4191.46674 | 885.602419 | 1198.53625 | 18.8510712 | 1.77690289 |
| p-p27 | 7.31495785 | 0.37818719 | 6757.56846 | 2555.62585 | 189.910928 | 9.03702438 | 2.4589372 |
| p38 | 6.46027583 | 0.25272441 | 3713.48359 | 938.487955 | 1043.38512 | 16.7504264 | 2.06378601 |
| p-p38 | 6.5446979 | 0.24457169 | 4408.19167 | 1078.1189 | 802.593762 | 16.2704786 | 1.94568151 |
| p-ERK | 6.44468899 | 0.20026886 | 4262.81051 | 853.708201 | 381.353761 | 7.32271088 | 1.84014502 |
| Cyclin B1 | 6.64415963 | 0.30382729 | 4395.02676 | 1335.32909 | 336.369724 | 7.53027933 | 2.883585 |
| IFN-y | 6.45587552 | 0.20864676 | 4468.47338 | 932.332503 | 208.361855 | 5.46462466 | 1.81229385 |
| biotin IFNy | 6.42640065 | 0.21276461 | 4075.07667 | 867.03208 | 443.965119 | 8.29371879 | 1.86209262 |
| 6A7 Bax | 6.69588997 | 0.28923639 | 4457.08591 | 1289.15144 | 389.869412 | 8.34131925 | 2.77285319 |
| Anti-BrdU | 7.87462317 | 1.04819122 | 9360.77698 | 9811.88428 | 17.6028326 | 3.91314999 | 6.89076795 |
| SR 1H4 | 6.43766881 | 0.28279005 | 3832.10196 | 1083.68032 | 1228.81542 | 23.5580461 | 1.95700576 |
| Clk1 | 6.45874743 | 0.20236299 | 4026.64226 | 814.843357 | 126.800971 | 2.54476741 | 1.92880376 |
| LnkB B2 | 6.76805579 | 0.32786603 | 4695.37197 | 1539.45295 | 418.000148 | 12.2604133 | 2.22320025 |
| Antibody | HT124K | HT124K | HT124K | HT124K | HT124K | HT124K | HT124K |
| Entropy | 95th%ile var | mean | stdev | kurtosis | skewness | DR | |
| AKT1(B-1) | 3.6388709 | 2.12000013 | 172.084868 | 364.819943 | 26306.4389 | 154.975997 | 2.53465347 |
| AKT1(7) | 4.33674097 | 1.01457968 | 236.252576 | 239.697063 | 57.2347386 | 5.49309364 | 6.84210526 |
| Akt2(D17) | 3.09156101 | 7.96233657 | 99.5903922 | 792.972222 | 5887.25715 | 74.611924 | 2.87755102 |
| p53 Ab1 | 4.09079536 | 2.71704953 | 260.076266 | 706.640097 | 7458.35971 | 83.2355533 | 2.8707483 |
| p53 Ab8 | 5.39118295 | 1.56358319 | 666.653349 | 1042.36797 | 428.188284 | 12.1835834 | 11.33 |
| DM1A | 3.72867736 | 2.82387858 | 142.715166 | 403.010301 | 18058.3269 | 127.906169 | 3.92753623 |
| JNK2 | 3.05772087 | 4.98656856 | 94.1531951 | 469.501363 | 17414.857 | 129.406235 | 2.64150943 |
| hnRNP-A1 | 3.74197272 | 1.10982143 | 169.803894 | 188.452 | 23264.3056 | 121.881413 | 3.09782609 |
| FDFT1 | 2.87791212 | 0.45522638 | 78.6092266 | 35.7849934 | 6202.12906 | 48.1048494 | 2.55319149 |
| p21 | 4.88056159 | 1.68084853 | 393.470917 | 661.365011 | 4580.52622 | 48.8499872 | 9.21804511 |
| p-p21 | 3.56907199 | 0.45462944 | 152.14079 | 69.1676822 | 149.452867 | 8.20823774 | 2.64044944 |
| p27 | 3.8794242 | 0.82617176 | 169.275086 | 139.850295 | 6939.03407 | 44.9492098 | 4.17647059 |
| p-p27 | 3.18314358 | 2.5335869 | 97.6363798 | 247.370252 | 53415.3941 | 221.336524 | 2.7962963 |
| p38 | 3.50561153 | 0.65966101 | 141.976199 | 93.6561625 | 20155.9907 | 96.2479979 | 2.71084337 |
| p-p38 | 3.64849878 | 1.7776175 | 161.28086 | 286.695679 | 37134.4212 | 182.895648 | 2.7311828 |
| p-ERK | 4.61384818 | 1.2271232 | 291.994153 | 358.3128 | 10823.1069 | 68.0910883 | 6.7800885 |
| Cyclin B1 | 3.26447123 | 2.69397794 | 104.030158 | 280.25495 | 36949.8652 | 182.207299 | 3 |
| IFN-y | 4.8490326 | 1.63623531 | 415.251753 | 679.449578 | 56.9535342 | 6.07266769 | 11.7723577 |
| biotin IFNy | 4.7279444 | 1.39308613 | 337.57957 | 470.277415 | 3010.19085 | 25.3004702 | 10.2293578 |
| 6A7 Bax | 4.44449254 | 1.48090943 | 230.309572 | 341.067618 | 6035.3077 | 57.5689551 | 8.11392405 |
| Anti-BrdU | 3.63407385 | 3.90077997 | 129.479389 | 505.070606 | 14506.1405 | 117.222898 | 3.67741935 |
| SR 1H4 | 3.2696181 | 1.79397273 | 131.299509 | 235.547738 | 51648.0352 | 205.542601 | 2.26829268 |
| Clk1 | 3.11953784 | 7.31745995 | 126.44449 | 925.252493 | 4679.6103 | 67.7634574 | 2.26760563 |
| LnkB B2 | 3.50192613 | 1.38543046 | 143.405965 | 198.678993 | 94781.8251 | 288.355356 | 2.67073171 |
| Antibody | CIM125K | CIM125K | CIM125K | CIM125K | CIM125K | CIM125K | CIM125K |
| Entropy | 95th%ile var | mean | stdev | kurtosis | skewness | DR | |
| AKT1(B-1) | 2.7304762 | 9.56982994 | 45.2389304 | 432.92887 | 21488.5224 | 144.901688 | 21.0285445 |
| AKT1(7) | 3.96246833 | 2.19201832 | 126.868797 | 278.098727 | 24682.4368 | 114.187669 | 23.6168285 |
| Akt2(D17) | 2.60484067 | 8.25812471 | 43.3103286 | 357.662095 | 29163.4835 | 166.275502 | 20.6748661 |
| p53 Ab1 | 2.80146844 | 2.8860048 | 44.1978863 | 127.555312 | 45700.074 | 189.930883 | 22.4974253 |
| p53 Ab8 | 3.26418745 | 4.88039295 | 68.4303708 | 333.967099 | 34156.1048 | 178.224829 | 19.630186 |
| DM1A | 2.88766904 | 1.64499373 | 53.2250516 | 87.5548762 | 12896.8206 | 73.1321188 | 27.4205021 |
| JNK2 | 2.98492852 | 1.89638275 | 54.1953277 | 102.775084 | 15832.7989 | 99.6436487 | 20.361336 |
| hnRNP-A1 | 3.50630798 | 2.41101314 | 90.1888438 | 217.446487 | 62303.2699 | 207.955481 | 28.9977876 |
| FDFT1 | 3.1782642 | 3.38281418 | 74.1013955 | 250.671251 | 43175.8734 | 180.552818 | 17.1889727 |
| p21 | 4.21970354 | 3.3232106 | 268.050998 | 890.789917 | 1227.63171 | 22.8112489 | 19.184719 |
| p-p21 | 3.39455331 | 1.19360386 | 78.1222364 | 93.2470027 | 7338.86079 | 45.3496167 | 30.2004608 |
| p27 | 4.55717389 | 2.17699657 | 250.41672 | 545.15634 | 212.4138 | 10.4702714 | 14.9222973 |
| p-p27 | 4.61752958 | 2.11107401 | 248.856763 | 525.355046 | 7398.48255 | 62.1267913 | 14.3288193 |
| p38 | 3.4471677 | 3.98325087 | 92.5377642 | 368.60113 | 12266.5409 | 91.6929588 | 27.7616279 |
| p-p38 | 2.80426187 | 7.66093095 | 69.5453262 | 532.781941 | 12536.7642 | 105.415318 | 24.4554335 |
| p-ERK | 3.29293995 | 5.27297172 | 71.8309949 | 378.762805 | 19803.3187 | 130.168903 | 21.3205298 |
| Cyclin B1 | 3.15329576 | 2.60616442 | 59.6286291 | 155.402012 | 93376.3096 | 281.279467 | 18.8500387 |
| IFN-y | 4.75572062 | 2.14758346 | 362.463633 | 778.420904 | 424.884236 | 9.97913068 | 16.8441989 |
| biotin IFNy | 2.77340883 | 14.9757514 | 68.9829021 | 1033.07079 | 3481.86931 | 58.0367062 | 20.5053191 |
| 6A7 Bax | 4.16781141 | 4.05548264 | 240.430834 | 975.063073 | 2365.29238 | 38.5653819 | 26.9137577 |
| Anti-BrdU | 3.61209451 | 5.95437037 | 88.4579233 | 526.711238 | 12990.5474 | 110.402725 | 23.5296572 |
| SR 1H4 | 3.00625783 | 1.5817682 | 56.1568498 | 88.8271193 | 24601.5136 | 108.767101 | 22.9143357 |
| Clk1 | 2.28663955 | 3.19223835 | 33.3028729 | 106.310708 | 36342.3158 | 165.64905 | 25.9708353 |
| LnkB B2 | 3.53470995 | 4.48848749 | 182.78519 | 820.429041 | 381.626817 | 15.7874471 | 17.5305079 |