PVH-OT neurons do not respond to sucrose consumption. A, B, Activity heatmaps for all PVH-OT neurons following the consumption of randomly delivered sucrose on (A) the first and (B) the second day of exposure. Each session consisted of 100 trials and the activity of each neuron was averaged across all trials for each session (3 mice, 105 neurons). C, On the first day of random sucrose delivery, none of the neurons showed positive responses to sucrose and 11% of neurons showed negative responses. D, On the second day of sucrose delivery, only 1% of neurons showed positive responses to sucrose and 3% showed negative responses.