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. 2020 Mar 11;40(11):2246–2258. doi: 10.1523/JNEUROSCI.2056-19.2020

Figure 6.

Figure 6.

Connexin hemichannel activity is reduced at 5 DPI. A, EtBr (4 μm) uptake in ependymal cells of the adult spinal cord (A1). Incubation of spinal cord slices with Cbx (200 μm) significantly reduced EtBr uptake (A2, p < 0.0001). B, The scrambled version of the Cx43 hemichannel specific blocker TAT-GAP19 did not affect EtBr uptake (B1, TAT-Gap19Scr), whereas the active peptide TAT-Gap19 (100 μm) significantly reduced EtBr uptake (B2, p < 0.0001). C, At 5 DPI, ependymal cells uptake less EtBr compared with the control. D1, Bar graphs represent the EtBr fluorescence units (FU) relative to the control conditions for each experiment (A,C,E). D2, Dot plots of FU in control, in the presence of TAT-Gap19Scr (100 μm) and at 5 DPI. There is a significant difference in connexin hemichannel activity between control and 5 DPI (p < 0.0001 control vs 5 DPI; p < 0.01 between TAT-Gap19 and 5 DPI; p = 0.99 between TAT-Gap19 and control). All images represent single optical sections. All data were obtained in C57BL/6J mice. **p < 0.01, ****p < 0.0001, ns = non significant.