CF-evoked complex spikes (CpSs) are altered in the Cacna2d2 KO, but intrinsic PC excitability is unchanged. A, Representative CF-evoked complex spikes in WT (left) and KO (right) PCs, arrow indicates CF stimulation. Each trace consists of 10 overlaid traces (lighter color) and the corresponding CpS average (dark color). B, Average number of spikelets per CpS in WT and KO PCs; **p < 0.01 (Mann–Whitney). C, Percentage of spikelets exceeding 30 mV trough-to-peak amplitude, ordered by spikelet number. D, Average CpS initial spike amplitude; p = 0.89 (NS). E, Average PC membrane potential when in zero current mode; p = 0.60 (NS). F, Representative single traces of membrane voltage responses to current injection, showing steps of 0, 100, 200 pA injections from Vm = −60 mV; left, WT (black); right, KO (green). Average Istep to initiate spiking WT = 200 ± 39 pA, n = 10; KO = 289 ± 82 pA, n = 9; p = 0.33 (NS). G, Average spike count during current steps from −0.2 to 1 nA, Vm = −60 mV. WT (black) and KO (green); p = 0.63 (NS; Two-way ANOVA with repeated measures, F(1,19) = 0.23). Data shown ± SEM, n = cells; unpaired Student's t test unless otherwise indicated.