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. Author manuscript; available in PMC: 2020 Mar 20.
Published in final edited form as: Ann Integr Mol Med. 2020;2(1):75–89. doi: 10.33597/aimm.02-1006

Figure 3: Projected topology of RNA-dependent generation of 5’-truncated mRNA encoding Aβ-containing C-terminal fragment of human beta-amyloid precursor protein.

Figure 3:

Lowercase letters -- nucleotide sequence of the antisense RNA. Uppercase letters -- nucleotide sequence of the sense RNA. Double-stranded portions highlighted in yellow: TCE (top) and ICE (bottom) elements of the antisense RNA. Note that the TCE and ICE are separated by about 2000 nucleotides. “2011–2013”: nucleotide positions on the antisense RNA (starting from the complement of the AUG encoding Met1 of the βAPP) of the “uac” (highlighted in blue) corresponding to the “AUG” (highlighted in green) encoding Met671 in the βAPP mRNA. a: TCE/ICE-guided folding of the antisense βAPP RNA. 3’-terminal “c” corresponds to one of multiple transcription start sites of βAPP mRNA located 149 nucleotides upstream from its AUG initiation codon [83]; note that such folding configuration would accommodate an additional 3’C, a transcript of the capG of βAPP mRNA (not shown). b: Extension of self-primed antisense RNA into sense RNA and cleavage (red arrow; may also occur at one of the TCE/ICE mismatches), after strand separation, of the chimeric intermediate. c: Chimeric RNA end product contains 5’terminal antisense segment extending into severely 5’-truncated βAPP mRNA. Its translation initiates from the “AUG” (highlighted in green and encoding Met671 in conventional βAPP mRNA) immediately preceding the beta amyloid-encoding region.