Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Caulobacter crescentus NA1000) | Caulobacter crescentus NA1000 | Evinger and Agabian; PMID:334726 | See Supplementary file 1 | |
| Antibody | CtrA Rrabbit polyclonal |
Delaby et al., 2019 PMID:31598724 | CtrA antibody are home-made raised against the full-length protein of C. crescentus. | Immunoblot: 1/5000 dilution. Figure 4—figure supplement 1 |
| Antibody | CitA Rrabbit polyclonal |
This study | CitA antibody are home-made raised against the full-length protein of C. crescentus. | Immunoblot: 1/5000 dilution Figure 4—figure supplement 1 |
| Antibody |
E. coli RNA Polymerase Antibody Sampler Kit Mouse monoclonal |
Biolegend | 699907 | Mix 1:1:1:1 between all sera. ChIP-Seq: 1/500 dilution, Figure 5 |
| Commercial assay or kit | Phos-tag | FUJIFILM Wako Chemicals | Distributor 300–93523 Manufacturer AAL-107M |
25 μM final, Figure 5 |
| Chemical compound | H332PO4 | Hartmann Analytic | Cat n° P-RB-1 | |
| Chemical compound | Acetonitrile OPTIMA LC/MS grade | Fisher Scientific | A955-212 | |
| Chemical compound | Methanol OPTIMA LC/MS grade | Fisher Scientific | A456-212 | |
| Chemical compound | Water LC/MS grade | Fisher Scientific | W/0112/17 | |
| Chemical compound | Formic acid | Biosolve | 069141 | |
| Chemical compound |
Ammonium hydroxide solution 25% | Sigma-Aldrich | 30501 | |
| Chemical compound | Mass Spectrometry Metabolite Library | Sigma-Aldrich | MSMLS-1EA | |
| Chemical compound | Major Mix IMS TOF calibration kit | Waters | 186008113 | |
| Chemical compound | Leucine Enkephalin | Waters | 700004768–1 | Waters TOF G2-S Sample Kit −2 (700008892) |
| Software, algorithm | UNIFI V.1.9.3 | Waters | ||
| Software, algorithm | Progenesis QI v2.3 | Nonlinear Dynamics, Waters | ||
| Software,algorithm | SIMCA-P 15.0 | Umetrics | ||
| Software, algorithm | MicrobeJ | Ducret, 2017 PMID:27572972 | ||
| Software, algorithm | SeqMonk | Babraham Bioinformatics Institute | V1.40.0 | |
| Other | Polyethyleneimine (PEI) plate | Sigma-Aldrich | Ref Z122882-25EA | Figure 6 |
| Other | Merck SeQuant ZIC-pHILIC column (150 × 2.1 mm, 5 µm) | Merck Millipore | 1504600001 | |
| Other | Merck SeQuant ZIC-pHILIC Guard kit (20 × 2.1 mm, 5 µm) | Merck Millipore | 1504380001 |