FIGURE 5.

Effects of 405 nm antimicrobial blue light (aBL) on platelet (PLT) activation in the absence or presence of agonists. PLT concentrates were irradiated with sham or 405 nm aBL at 75 J/cm² once and then evaluated for PLT activations in 2 hours (A), 2 days (B) or 3 days(C) after irradiation. Alternatively, the PLT concentrate was irradiated once a day for three consecutive days and determined for PLT activation in2 hours after the final irradiation (D). Percentages of PLTs positive for CD62P or GPIIb/IIIa expression in the absence or presence of an indicated agonist were evaluated by flow cytometry. The agonists tested were TRAP-6 at 10 μM, thrombin at 2 U/mL or ADP at 50 μM. Data represent mean ± SEM of six independent experiments with each assayed in triplicate. ns, no significance between sham- and aBL-treated samples