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. Author manuscript; available in PMC: 2020 Mar 20.
Published in final edited form as: J Biophotonics. 2019 Aug 29;13(1):e201960021. doi: 10.1002/jbio.201960021

FIGURE 7.

FIGURE 7

Antimicrobial blue light (aBL) did not affect recovery of mouse platelets (PLTs) following transfusion into mice. Concentrated PLTs were freshly prepared from pooled mouse blood samples, treated with sham or 405 nm aBL at 75 J/cm2 and stored for 2 hours (A and B) or5 days (C and D), after which the PLTs were labeled with calcein and intravenously infused into recipient mice. Blood was drawn at indicated times after infusion in alternative mice, labeled with anti-CD41 and analyzed by flow cytometry. A and C are representative dot plots of 2-hour- (A) and 5-day-storaged (C) PLTs from recipients labeled with calcein and CD41 at different time points after transfusion. B and D are changes of percentage double-labeled events in sham- and aBL irradiated-PLTs at indicated times after 2 hours (B) or 5 days (D) of storage. Results are shown as percent double-colored PLTs ± SEM with five mice in each time point