Table 1.
The composition of Dulbecco's modified Eagle's medium - high glucose (DMEM-H).
| Composition | Molecular weight | Content, mg/L |
|---|---|---|
| Inorganic salts | ||
| CaCl2 | 111 | 200.00 |
| Fe(NO3)3•9H2O | 404 | 0.10 |
| KCl | 75 | 400.00 |
| MgSO4 | 120 | 97.67 |
| Salt | 58 | 6,400.00 |
| NaH2PO4 | 138 | 125.00 |
| NaHCO3 | 84.0 | 3700.0 |
| Vitamins | ||
| Choline chloride | 140 | 4.00 |
| D-calcium pantothenate | 477 | 4.00 |
| Folic acid | 441 | 4.00 |
| Niacinamide | 122 | 4.00 |
| Riboflavin | 376 | 0.40 |
| Thiamine hydrochloride | 337 | 4.00 |
| i-inositol | 180 | 7.20 |
| Pyridoxine hydrochloride | 206 | 4.00 |
| Others | ||
| D-Glucose | 180 | 4,500.00 |
| Sodium pyruvate | 110 | 110.00 |
| Phenol red | 376 | 15.00 |
| Amino acids1 | ||
| Glycine | 75 | 30.00 |
| L-arginine hydrochloride | 211 | 84.00 |
| L-cystine | 240 | 48.00 |
| L-glutamine | 146 | 584.00 |
| L-histidine hydrochloride-H2O | 210 | 42.00 |
| L-isoleucine | 131 | 105.00 |
| L-leucine | 131 | 105.00 |
| L-lysine hydrochloride | 183 | 146.00 |
| L-methionine | 149 | 30.00 |
| L-phenylalanine | 165 | 66.00 |
| L-serine | 105 | 42.00 |
| L-threonine | 119 | 95.00 |
| L-tryptophan | 204 | 16.00 |
| L-tyrosine disodium salt hydrate | 225 | 90.00 |
| L-valine | 117 | 94.00 |
1
The content of amino acids is for 1AA group (1-fold AA medium).