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. 2020 Mar 20;11:1494. doi: 10.1038/s41467-020-15188-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Heatmap showing normalized mRNA expression of genes encoding secreted proteins that are induced in fibroblasts from MDA-LM2 micrometastasis and further induced in MDA- and MDA-LM2 macrometastasis. b RT-qPCR validation of Cxcl9 and Cxcl10 induction in isolated fibroblasts from MDA- and MDA-LM2-derived lung metastases compared with expression in fibroblasts from healthy lungs. Shown are means from three mice per group with SD. c Cxcl9 and Cxcl10 expression analyzed by RT-qPCR in indicated cell types isolated from MDA-LM2-macrometastatic lungs relative to overall expression in whole lungs. Data show mean from four mice with SD. P values were calculated by two-way ANOVA with Tukey’s multiple comparisons test to compare Cxcl9/10 expression in all indicated cell types. Shown are summarized P values of Cxcl9/10 upregulation in fibroblasts compared with all other cell types. ** P < 0.01, **** P < 0.0001. Individual P values for expression of Cxcl9/Cxcl10 in fibroblasts versus indicated cell populations are: whole lung: P < 0.0001/P = 0.0048, endothelial cells: P < 0.0001/P < 0.0001, hematopoietic cells: P < 0.0001/P = 0.0080, epithelial cells: P < 0.0001/P < 0.0001. P values in all other comparisons were not significant (P > 0.05). d Correlation analysis of CXCL9 and CXCL10 expression in dissected human metastases from breast cancer patients (GSE14020). Linear regression with Pearson correlation r and two-tailed P value, n = 65. e Oncosphere formation of MDA or SUM breast cancer cells overexpressing CXCL9/10 or a vector control. Data represent five and four independent experiments, respectively, with quantification of ten wells per condition. Boxes depict median with upper and lower quartiles. Data points show values of biological replicates and whiskers indicate minimum and maximum values. P values were calculated by unpaired two-tailed t-tests on biological replicates.