Table 1.
Summary of binding lifetimes of wild-type and mutant UvrA-YPet in the absence of exogenous DNA damage.
| Strain (genotype) | Probe | Treatment | Afast (%) | τfast (s) | Aslow (%) | τslow (s) | kb (s−1) | Exponential fit | Figure |
|---|---|---|---|---|---|---|---|---|---|
| ΔuvrA ΔuvrB Δmfd/pUvrA-YPet | UvrA-YPet (plasmid) | – | 72 ± 2 | 1.6 ± 0.1 | 28 ± 2 | 24 ± 1 | 5.5 ± 0.1 | 2 | 3 |
| uvrA-YPet ΔuvrB Δmfd | UvrA-YPet (chr) | – | 73 ± 4 | 1.4 ± 0.4 | 27 ± 4 | 22 ± 8 | 5.5 ± 0.5 | 2 | 3 |
| ΔuvrA/ pUvrA(Δ131–250)-YPet | UvrA(Δ131–250)-YPet (plasmid) | – | 100 | 29.7 ± 0.8 | 5.2 ± 0.05 | 1 | 3 | ||
| uvrA-YPet Δmfd | UvrA-YPet (chr) | – | 78 ± 2 | 1.5 ± 0.1 | 22 ± 2 | 8.7 ± 0.4 | 6.3 ± 0.2 | 2 | 3 |
| uvrA-YPet uvrB(ΔβHG) Δmfd | UvrA-YPet (chr) | – | 100 | 148 ± 36 | 6.0 ± 0.2 | 1 | 3 | ||
| uvrA-YPet ΔuvrB | UvrA-YPet (chr) | – | – | – | 100 | 97 ± 18 | 7.3 ± 0.1 | 1 | 3 |
| uvrA-YPet | UvrA-YPet (chr) | – | 79 ± 2 | 1.9 ± 0.2 | 21 ± 2 | 12.0 ± 0.8 | 7.2 ± 0.2 | 2 | 4 |
| uvrA-YPet | UvrA-YPet (chr) | rif | 63 ± 3 | 1.5 ± 0.3 | 37 ± 3 | 9.6 ± 0.6 | 5.7 ± 0.2 | 2 | 4 |
| ΔuvrA/pUvrA-YPet | UvrA-YPet (plasmid) | – | 74 ± 2 | 2.0 ± 0.1 | 26 ± 2 | 19 ± 1 | 6.8 ± 0.1 | 2 | 4 |
| ΔuvrA/pUvrA-YPet | UvrA-YPet (plasmid) | rif | 75 ± 2 | 1.7 ± 0.1 | 25 ± 2 | 11.5 ± 0.6 | 7.3 ± 0.1 | 2 | 4 |